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James M Wagner
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Synthetic biology and molecular genetics in non-conventional yeasts: current tools and future advances
JM Wagner, HS Alper
Fungal Genetics and Biology 89, 126-136, 2016
2082016
Rewiring Yarrowia lipolytica toward triacetic acid lactone for materials generation
KA Markham, CM Palmer, M Chwatko, JM Wagner, C Murray, S Vazquez, ...
Proceedings of the National Academy of Sciences 115 (9), 2096-2101, 2018
1662018
Compartmentalized microbes and co-cultures in hydrogels for on-demand bioproduction and preservation
TG Johnston, SF Yuan, JM Wagner, X Yi, A Saha, P Smith, A Nelson, ...
Nature Communications 11 (1), 563, 2020
1592020
In vivo continuous evolution of genes and pathways in yeast
N Crook, J Abatemarco, J Sun, JM Wagner, A Schmitz, HS Alper
Nature communications 7 (1), 13051, 2016
1352016
RNA-aptamers-in-droplets (RAPID) high-throughput screening for secretory phenotypes
J Abatemarco, MF Sarhan, JM Wagner, JL Lin, L Liu, W Hassouneh, ...
Nature communications 8 (1), 332, 2017
1322017
Biosensor‐Enabled Directed Evolution to Improve Muconic Acid Production in Saccharomyces cerevisiae
JM Leavitt, JM Wagner, CC Tu, A Tong, Y Liu, HS Alper
Biotechnology journal 12 (10), 1600687, 2017
1232017
Enabling tools for high-throughput detection of metabolites: metabolic engineering and directed evolution applications
JL Lin, JM Wagner, HS Alper
Biotechnology advances 35 (8), 950-970, 2017
1102017
A comparative analysis of single cell and droplet-based FACS for improving production phenotypes: riboflavin overproduction in Yarrowia lipolytica
JM Wagner, L Liu, SF Yuan, MV Venkataraman, AR Abate, HS Alper
Metabolic engineering 47, 346-356, 2018
932018
Validating genome-wide CRISPR-Cas9 function improves screening in the oleaginous yeast Yarrowia lipolytica
C Schwartz, JF Cheng, R Evans, CA Schwartz, JM Wagner, S Anglin, ...
Metabolic engineering 55, 102-110, 2019
852019
Developing a piggyBac Transposon System and Compatible Selection Markers for Insertional Mutagenesis and Genome Engineering in Yarrowia lipolytica
JM Wagner, EV Williams, HS Alper
Biotechnology journal 13 (5), 1800022, 2018
692018
T7 Polymerase Expression of Guide RNAs in vivo Allows Exportable CRISPR-Cas9 Editing in Multiple Yeast Hosts
NJ Morse, JM Wagner, KB Reed, MR Gopal, LH Lauffer, HS Alper
ACS synthetic biology 7 (4), 1075-1084, 2018
602018
Enhanced production of Chikungunya virus-like particles using a high-pH adapted Spodoptera frugiperda insect cell line
JM Wagner, JD Pajerowski, CL Daniels, PM McHugh, JA Flynn, JW Balliet, ...
PloS one 9 (4), e94401, 2014
532014
Yeast Terminator Function Can Be Modulated and Designed on the Basis of Predictions of Nucleosome Occupancy
NJ Morse, MR Gopal, JM Wagner, HS Alper
ACS synthetic biology 6 (11), 2086-2095, 2017
342017
Sorting for secreted molecule production using a biosensor-in-microdroplet approach
EK Bowman, JM Wagner, SF Yuan, M Deaner, CM Palmer, S D’Oelsnitz, ...
Proceedings of the National Academy of Sciences 118 (36), e2106818118, 2021
232021
Design, Evolution, and Characterization of a Xylose Biosensor in Escherichia coli Using the XylR/xylO System with an Expanded Operating Range
RQ Tang, JM Wagner, HS Alper, XQ Zhao, FW Bai
ACS Synthetic Biology 9 (10), 2714-2722, 2020
192020
Improving ionic liquid tolerance in Saccharomyces cerevisiae through heterologous expression and directed evolution of an ILT1 homolog from Yarrowia lipolytica
KB Reed, JM Wagner, S d’Oelsnitz, JM Wiggers, HS Alper
Journal of Industrial Microbiology and Biotechnology 46 (12), 1715-1724, 2019
162019
Design and Evaluation of Synthetic Terminators for Regulating Mammalian Cell Transgene Expression
JK Cheng, NJ Morse, JM Wagner, SK Tucker, HS Alper
ACS synthetic biology 8 (6), 1263-1275, 2019
102019
Spectroscopic analysis of chlamydial major outer membrane protein in support of structure elucidation
RW Hepler, DD Nahas, B Lucas, R Kaufhold, JA Flynn, JD Galli, ...
Protein Science 27 (11), 1923-1941, 2018
72018
Rapid In-Process Measurement of Live Virus Vaccine Potency Using Laser Force Cytology: Paving the Way for Rapid Vaccine Development
R McCracken, N Al-Nazal, T Whitmer, S Yi, JM Wagner, CG Hebert, ...
Vaccines 10 (10), 1589, 2022
42022
Genome Engineering of Yarrowia lipolytica with the PiggyBac Transposon System.
JM Wagner, CM Palmer, MV Venkataraman, LH Lauffer, JM Wiggers, ...
Methods in Molecular Biology (Clifton, NJ) 2307, 1-24, 2021
32021
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